Abstract
Vaults are ubiquitous, self-assembled protein nanocapsules with dimension in the sub-100 nm range that are conserved across diverse phyla from worms to humans. Their normal presence in humans at a copy number of over 10,000/cell makes them attractive as potential drug delivery vehicles. Toward this goal, bifunctional amine-reactive reagents are shown to be useful for the reversible cross-linking of recombinant vaults such that they may be closed and opened in a controllable manner.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amines / chemistry
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Animals
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Biotechnology / methods
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Chemistry, Pharmaceutical / methods
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Cross-Linking Reagents / chemistry
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Drug Delivery Systems
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Electrons
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Humans
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Hydrogen-Ion Concentration
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Models, Chemical
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Molecular Conformation
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Nanocapsules / chemistry*
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Nanoparticles / chemistry*
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Nanotechnology / methods*
Substances
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Amines
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Cross-Linking Reagents
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Nanocapsules