Applications of next-generation sequencing technologies in functional genomics

Genomics. 2008 Nov;92(5):255-64. doi: 10.1016/j.ygeno.2008.07.001. Epub 2008 Aug 24.

Abstract

A new generation of sequencing technologies, from Illumina/Solexa, ABI/SOLiD, 454/Roche, and Helicos, has provided unprecedented opportunities for high-throughput functional genomic research. To date, these technologies have been applied in a variety of contexts, including whole-genome sequencing, targeted resequencing, discovery of transcription factor binding sites, and noncoding RNA expression profiling. This review discusses applications of next-generation sequencing technologies in functional genomics research and highlights the transforming potential these technologies offer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biotechnology / methods*
  • Computational Biology / methods*
  • Gene Expression Profiling*
  • Genome, Human
  • Genomics*
  • Humans
  • Sequence Analysis, DNA / instrumentation
  • Sequence Analysis, DNA / methods*