Distribution of six oncogenic types of human papillomavirus and type 16 integration analysis in Chinese women with cervical precancerous lesions and carcinomas

Tumour Biol. 2008;29(2):105-13. doi: 10.1159/000135690. Epub 2008 Jun 2.

Abstract

Objectives: The prevalent genotypes of human papillomavirus (HPV) infection and the significance of HPV16 integration in cervical precancerous lesions and cancer of Chinese women were investigated.

Methods: HPV genotyping and HPV16 integration status were studied on 100 normal cervical cytology, 90 low-grade (LSIL) and 99 high-grade squamous intraepithelial lesions (HSIL), as well as 96 cervical cancer biopsies using DNA sequencing and quantitative real-time PCR.

Results: HPV were detected in 12.0% of normal cytology, 93.3% of LSIL, 90.9% of HSIL and 89.6% of cervical cancer samples. High-risk HPV16, 18, 58, 52, 33 and 31 were the 6 major HPV types observed, composing 45.6, 82.8 and 85.4% of HPV infections in LSIL, HSIL and cervical cancers, respectively, with a significant rising trend in increasing disease severity (p = 0.002). While HPV16 and HPV18 were most commonly found in cervical cancer, HPV58 was prevalently observed in cervical precancerous lesions, and its frequency decreased with increased disease severity. Among HPV16-positive patients, 40.0% of LSIL, 88.9% of HSIL and 86.4% of cancer cases had HPV16 integration. The frequency of the integrated form of HPV16 significantly increased with the severity of the disease.

Conclusions: Our results demonstrated that high-risk HPV infection and viral DNA integration were in progressive frequency from low-grade cervical precancerous lesions to cancer. HPV16 integration status had the potential to be a marker for cancer risk assessment of cervical precancerous lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Biomarkers, Tumor / genetics
  • Carcinoma, Squamous Cell / ethnology
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / virology
  • Case-Control Studies
  • Cervix Uteri / virology
  • China
  • DNA, Viral / genetics
  • Female
  • Genes, Viral / genetics*
  • Genotype
  • Human papillomavirus 16 / genetics*
  • Human papillomavirus 18 / genetics
  • Humans
  • Middle Aged
  • Oncogenes / genetics*
  • Papillomavirus Infections / ethnology
  • Papillomavirus Infections / genetics
  • Precancerous Conditions / ethnology
  • Precancerous Conditions / genetics
  • Precancerous Conditions / virology*
  • Prevalence
  • Uterine Cervical Dysplasia / ethnology
  • Uterine Cervical Dysplasia / genetics
  • Uterine Cervical Dysplasia / virology*
  • Uterine Cervical Neoplasms / ethnology
  • Uterine Cervical Neoplasms / genetics
  • Uterine Cervical Neoplasms / virology*
  • Virus Integration / genetics*

Substances

  • Biomarkers, Tumor
  • DNA, Viral