Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product

D P Bottaro; J S Rubin; D L Faletto; A M Chan; T E Kmiecik; G F Vande Woude; S A Aaronson

doi:10.1126/science.1846706

Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product

Science. 1991 Feb 15;251(4995):802-4. doi: 10.1126/science.1846706.

Authors

D P Bottaro¹, J S Rubin, D L Faletto, A M Chan, T E Kmiecik, G F Vande Woude, S A Aaronson

Affiliation

¹ Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.

PMID: 1846706
DOI: 10.1126/science.1846706

Abstract

Hepatocyte growth factor (HGF) is a plasminogen-like protein thought to be a humoral mediator of liver regeneration. A 145-kilodalton tyrosyl phosphoprotein observed in rapid response to HGF treatment of intact target cells was identified by immunoblot analysis as the beta subunit of the c-met proto-oncogene product, a membrane-spanning tyrosine kinase. Covalent cross-linking of 125I-labeled ligand to cellular proteins of appropriate size that were recognized by antibodies to c-met directly established the c-met product as the cell-surface receptor for HGF.

MeSH terms

Cell Line
Cross-Linking Reagents
Growth Substances / metabolism*
Growth Substances / physiology
Hepatocyte Growth Factor
Humans
Molecular Weight
Phosphorylation
Precipitin Tests
Protein-Tyrosine Kinases / metabolism*
Proto-Oncogene Mas
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-met
Receptors, Cell Surface / metabolism*

Substances

Cross-Linking Reagents
Growth Substances
MAS1 protein, human
Proto-Oncogene Mas
Proto-Oncogene Proteins
Receptors, Cell Surface
Hepatocyte Growth Factor
Protein-Tyrosine Kinases
Proto-Oncogene Proteins c-met