A highly specific BODIPY-based fluorescent probe for the detection of hypochlorous acid

Zhen-Ning Sun; Feng-Qin Liu; Yan Chen; Paul Kwong Hang Tam; Dan Yang

doi:10.1021/ol800507m

A highly specific BODIPY-based fluorescent probe for the detection of hypochlorous acid

Org Lett. 2008 Jun 5;10(11):2171-4. doi: 10.1021/ol800507m. Epub 2008 May 1.

Authors

Zhen-Ning Sun¹, Feng-Qin Liu, Yan Chen, Paul Kwong Hang Tam, Dan Yang

Affiliation

¹ Department of Chemistry, The University of Hong Kong, Hong Kong, P.R. China.

PMID: 18447382
DOI: 10.1021/ol800507m

Abstract

A fluorescent probe, HKOCl-1, has been successfully developed for the detection of hypochlorous acid on the basis of a specific reaction with p-methoxyphenol. The formation of HOCl has been successfully detected not only in an abiotic system but also in an enzymatic system (myeloperoxidase/H2O2/Cl(-) system) and in living macrophage cells upon stimulation. This new probe might be used as an efficient tool for probing the roles HOCl plays in biological systems.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Boron Compounds / chemistry*
Fluorescence
Fluorescent Dyes / chemistry*
Hypochlorous Acid / analysis*
Hypochlorous Acid / chemistry*
Substrate Specificity

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Fluorescent Dyes
Hypochlorous Acid