Stabilization of beta-propeller phytase by introducing Xaa-->Pro and Gly-->Ala substitutions at consensus positions

Protein Pept Lett. 2008;15(3):297-9. doi: 10.2174/092986608783744216.

Abstract

Phytases play important roles in agricultural and feed industries. In this study, the stability of a beta-propeller phytase, PhyL, from Bacillus licheniformis was successfully improved by introducing Xaa-->Pro and Gly-->Ala substitutions at consensus positions. Our results suggest that Gly-->Ala substitution is a more promising strategy to improve protein stability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 6-Phytase / chemistry*
  • Alanine / chemistry*
  • Amino Acid Sequence
  • Amino Acid Substitution
  • Bacillus / enzymology
  • Consensus Sequence
  • Glycine / chemistry*
  • Molecular Sequence Data
  • Proline / chemistry*
  • Protein Structure, Tertiary
  • Sequence Alignment

Substances

  • Proline
  • 6-Phytase
  • Alanine
  • Glycine