Flow cytometric measurement of cell cycle kinetics in rat Walker-256 carcinoma following in vivo and in vitro pulse labelling with bromodeoxyuridine

Cytometry. 1991;12(1):33-41. doi: 10.1002/cyto.990120106.

Abstract

Flow cytometric measurements of total DNA content, cell cycle distribution, and bromodeoxyuridine (BrdUrd) uptake were made in rat Walker-256 carcinoma cells. After both in vivo and in vitro pulse labelling with BrdUrd, Walker-256 tumor cells were stained with propidium iodide (PI) to estimate the total DNA content and a monoclonal antibody against BrdUrd to estimate the relative amount of cells in S phase. BrdUrd-labelled single cell suspensions were harvested at different time intervals to determine the movement of these cells within the cell cycle. To increase BrdUrd uptake, fluorodeoxyuridine (FDU), a thymidine antagonist, was also applied in vivo and in vitro. The results indicated exponential growth characteristics for this tumor between days 5 and 8 after implantation. Tumor doubling times, derived from changes in tumor volume in vivo and from the increase in cell number in vitro were similar. The mean time for DNA synthesis was estimated from the relative movement of BrdUrd-labelled cells towards G2. The percent of cells labelled with BrdUrd and the DNA synthesis time were similar regardless of the mode of BrdUrd administration. This study demonstrates that BrdUrd labelling of rat Walker-256 carcinoma cells in vitro yields kinetic estimates of tumor proliferation during exponential growth similar to those with the administration of BrdUrd in the intact tumor-bearing rat.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bromodeoxyuridine* / metabolism
  • Bromodeoxyuridine* / pharmacokinetics
  • Carcinoma 256, Walker / chemistry
  • Carcinoma 256, Walker / metabolism
  • Carcinoma 256, Walker / pathology*
  • Cell Cycle / physiology*
  • Cell Line
  • Cell Transformation, Neoplastic / metabolism
  • Cell Transformation, Neoplastic / pathology
  • DNA / analysis
  • DNA / metabolism
  • Flow Cytometry / methods*
  • Floxuridine
  • G1 Phase / physiology
  • G2 Phase / physiology
  • Male
  • Mathematics
  • Models, Biological
  • Propidium
  • Rats
  • Rats, Inbred Strains
  • S Phase / physiology
  • Time Factors
  • Tumor Cells, Cultured / chemistry
  • Tumor Cells, Cultured / metabolism
  • Tumor Cells, Cultured / pathology

Substances

  • Floxuridine
  • Propidium
  • DNA
  • Bromodeoxyuridine