In vivo coronary artery imaging remains technically difficult in mice and only a few studies have been reported so far. 3D method is usually time-consuming and presents a practical limitation for MRI study of coronary arteries in mouse models of various cardiovascular diseases. The current study aims to develop a rapid and robust 2D procedure to visualize both LCA and RCA of mice in vivo. In addition, contrast agent of Gd-DPTA by rapid intraperitoneal (i.p.) injection was examined for its ability to improve the quality of LCA and RCA visualization. The current study demonstrated the feasibility of rapid MRI examination of coronary arteries of mice in vivo using 2D method and i.p. injection of contrast agent.