Investigation of infarct myocardium structure will lead to better understanding of functional adaptation and remodeling. Diffusion tensor magnetic resonance imaging (DTI) provides a means for rapid and nondestructive characterization of the three-dimensional fiber architecture of myocardium. DTI studies were performed on 10 excised, formalin-fixed hearts of both infarct (two months after left anterior descending coronary artery (LAD) occlusion surgery, n=4) and control (n=6) porcine. Each slice was divided into eight segments, and fractional anisotropy (FA) value and helix angle were measured in multiples short-axis slices, respectively. Infarct myocardium exhibited decreased FA value, flatter helix angle courses fluctuating around small helix angle with greater standard error of the mean (SEM) and smaller range of helix angle. The results provide structure information of infarct myocardium.