Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis

Biotechnol Lett. 2007 Oct;29(10):1575-82. doi: 10.1007/s10529-007-9447-x. Epub 2007 Jul 4.

Abstract

Among 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Helicases / genetics
  • DNA-Binding Proteins / genetics
  • Escherichia coli / genetics
  • Genetic Vectors / genetics*
  • Humans
  • Molecular Sequence Data
  • Neisseriaceae / genetics*
  • Open Reading Frames / genetics
  • Plasmids / genetics*
  • Replication Origin / genetics
  • Trans-Activators / genetics
  • Transformation, Bacterial

Substances

  • DNA-Binding Proteins
  • Trans-Activators
  • replication initiator protein
  • DNA Helicases