Abstract
Using serum from guinea-pigs immunized with a DeltaAFMP1DeltaAFMP2 deletion mutant of Aspergillus fumigatus to screen a cDNA library of A. fumigatus, we cloned a novel immunogenic 57-kDa protein in A. fumigatus. We also cloned its 55-kDa homologue in Penicillium marneffei, which was possibly related to amino acid biosynthesis and metabolism, with homologues present only in the subphylum Pezizomycotina of Ascomycota. The recombinant 55-kDa protein of P. marneffei reacted strongly with guinea-pig serum immunized with P. marneffei and with the sera of patients with P. marneffei infection. A similar approach could be applied to immunogenic protein screening in other microorganisms for serological diagnosis, epidemiological studies and the study of vaccines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Fungal / immunology
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Antigens, Fungal / genetics
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Aspergillus fumigatus / genetics*
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Aspergillus fumigatus / immunology*
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Base Sequence
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Blotting, Southern
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Blotting, Western
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Bone Marrow Transplantation
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Cloning, Molecular
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Fungal Proteins / genetics
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Fungal Proteins / immunology*
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Fungal Proteins / isolation & purification
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Gene Deletion
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Genes, Fungal
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Guinea Pigs
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Humans
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Immunologic Techniques*
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Membrane Glycoproteins / genetics
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Molecular Sequence Data
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Mycoses / microbiology*
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Penicillium / classification
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Penicillium / immunology*
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Penicillium / isolation & purification*
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Phylogeny
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Recombinant Proteins / immunology
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Recombinant Proteins / isolation & purification
Substances
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Antibodies, Fungal
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Antigens, Fungal
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Fungal Proteins
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Membrane Glycoproteins
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Recombinant Proteins
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galactomannoprotein, Aspergillus fumigatus
Associated data
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GENBANK/DQ009009
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GENBANK/DQ009010