Molecular characterization of isoniazid resistance in Mycobacterium tuberculosis: identification of a novel mutation in inhA

E T Y Leung; P L Ho; K Y Yuen; W L Woo; T H Lam; R Y Kao; W H Seto; W C Yam

doi:10.1128/AAC.50.3.1075-1078.2006

Molecular characterization of isoniazid resistance in Mycobacterium tuberculosis: identification of a novel mutation in inhA

Antimicrob Agents Chemother. 2006 Mar;50(3):1075-8. doi: 10.1128/AAC.50.3.1075-1078.2006.

Authors

E T Y Leung¹, P L Ho, K Y Yuen, W L Woo, T H Lam, R Y Kao, W H Seto, W C Yam

Affiliation

¹ Department of Microbiology, 4/F, University Pathology Bldg., Queen Mary Hospital, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

Abstract

Multiplex allele-specific PCRs detecting katG codon 315 and mabA (bp -15) mutations could specifically identify 77.5% of isoniazid-resistant Mycobacterium tuberculosis strains in the South China region. One clinical isolate harboring InhA Ile194Thr was characterized to show strong association with isoniazid resistance in Mycobacterium tuberculosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Antitubercular Agents / pharmacology*
Bacterial Proteins / genetics*
Catalase / genetics
Catalase / metabolism
Codon
DNA, Bacterial / analysis
DNA, Bacterial / genetics
Drug Resistance, Bacterial / genetics
Genes, Bacterial*
Humans
Isoniazid / pharmacology*
Kinetics
Microbial Sensitivity Tests
Mycobacterium tuberculosis / drug effects
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / isolation & purification
Peroxidases / genetics
Point Mutation*
Polymerase Chain Reaction
Sequence Analysis, DNA

Substances

Antitubercular Agents
Bacterial Proteins
Codon
DNA, Bacterial
Peroxidases
Catalase
Isoniazid