Application of immobilized metal affinity chromatography in proteomics

Expert Rev Proteomics. 2005 Oct;2(5):649-57. doi: 10.1586/14789450.2.5.649.

Abstract

It has been proved that the progress of proteomics is mostly determined by the development of advanced and sensitive protein separation technologies. Immobilized metal affinity chromatography (IMAC) is a powerful protein fractionation method used to enrich metal-associated proteins and peptides. In proteomics, IMAC has been widely employed as a prefractionation method to increase the resolution in protein separation. The combination of IMAC with other protein analytical technologies has been successfully utilized to characterize metalloproteome and post-translational modifications. In the near future, newly developed IMAC integrated with other proteomic methods will greatly contribute to the revolution of expression, cell-mapping and structural proteomics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Chemical Fractionation
  • Chromatography, Affinity / methods*
  • Humans
  • Metals / chemistry*
  • Proteins / chemistry
  • Proteomics / methods*

Substances

  • Metals
  • Proteins