Abstract
We developed a sensitive and rapid real-time reverse transcription-polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples. This assay was evaluated with samples from H5N1-infected patients and demonstrated greater sensitivity and faster turnaround time than nested RT-PCR.
MeSH terms
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Antigens, Viral / chemistry
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Antigens, Viral / genetics
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Hemagglutinins / chemistry
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Hemagglutinins / genetics
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Humans
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Influenza A Virus, H5N1 Subtype*
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Influenza A virus / genetics
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Influenza A virus / isolation & purification*
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Influenza, Human / diagnosis
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Influenza, Human / virology*
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RNA, Viral / chemistry
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RNA, Viral / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Sensitivity and Specificity
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Time Factors
Substances
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Antigens, Viral
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Hemagglutinins
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RNA, Viral