Influenza A H5N1 detection

Emerg Infect Dis. 2005 Aug;11(8):1303-5. doi: 10.3201/eid1108.041317.

Abstract

We developed a sensitive and rapid real-time reverse transcription-polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples. This assay was evaluated with samples from H5N1-infected patients and demonstrated greater sensitivity and faster turnaround time than nested RT-PCR.

MeSH terms

  • Antigens, Viral / chemistry
  • Antigens, Viral / genetics
  • Hemagglutinins / chemistry
  • Hemagglutinins / genetics
  • Humans
  • Influenza A Virus, H5N1 Subtype*
  • Influenza A virus / genetics
  • Influenza A virus / isolation & purification*
  • Influenza, Human / diagnosis
  • Influenza, Human / virology*
  • RNA, Viral / chemistry
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Time Factors

Substances

  • Antigens, Viral
  • Hemagglutinins
  • RNA, Viral