SARS coronavirus detection methods

Susanna K P Lau; Xiao-Yan Che; Patrick C Y Woo; Beatrice H L Wong; Vincent C C Cheng; Gibson K S Woo; Ivan F N Hung; Rosana W S Poon; Kwok-Hung Chan; J S Malik Peiris; Kwok-Yung Yuen

doi:10.3201/eid1107.041045

SARS coronavirus detection methods

Emerg Infect Dis. 2005 Jul;11(7):1108-11. doi: 10.3201/eid1107.041045.

Authors

Susanna K P Lau¹, Xiao-Yan Che, Patrick C Y Woo, Beatrice H L Wong, Vincent C C Cheng, Gibson K S Woo, Ivan F N Hung, Rosana W S Poon, Kwok-Hung Chan, J S Malik Peiris, Kwok-Yung Yuen

Affiliation

¹ Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China.

Abstract

Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription-polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Enzyme-Linked Immunosorbent Assay* / methods
Feces / virology
Humans
Nucleocapsid Proteins / isolation & purification
Reverse Transcriptase Polymerase Chain Reaction / methods
Sensitivity and Specificity
Severe Acute Respiratory Syndrome / diagnosis*
Severe Acute Respiratory Syndrome / urine
Severe acute respiratory syndrome-related coronavirus / isolation & purification*

Substances

Nucleocapsid Proteins