Abstract
Previous studies showed that electroacupuncture (EA) significantly attenuates inflammatory hyperalgesia in a complete Freund's adjuvant (CFA)-induced inflammatory pain rat model. The present study demonstrates that pretreatment with Derm-sap, a selective toxin for neurons that contain mu opioid receptor (MOR), specifically decreases MOR and blocks EA anti-hyperalgesia. These data suggest that spinal MOR-containing neurons are involved in the processes by which EA produces anti-hyperalgesia.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Analysis of Variance
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Animals
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Blotting, Western / methods
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Electroacupuncture / methods*
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Freund's Adjuvant / adverse effects
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Hindlimb / drug effects
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Hindlimb / innervation*
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Hyperalgesia / etiology
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Hyperalgesia / therapy*
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Immunohistochemistry / methods
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Inflammation / chemically induced
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Inflammation / complications
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Lectins / metabolism
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Male
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N-Glycosyl Hydrolases
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Neurons / metabolism*
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Opioid Peptides
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Pain Measurement / methods
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Reaction Time / drug effects
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Receptors, Opioid, delta / metabolism
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Receptors, Opioid, mu / metabolism*
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Recombinant Fusion Proteins / pharmacology
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Ribosome Inactivating Proteins, Type 1
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Saporins
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Skin / drug effects
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Skin / innervation
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Spinal Cord / cytology*
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Spinal Cord / drug effects
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Spinal Cord / metabolism
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Substance P / metabolism
Substances
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Lectins
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Opioid Peptides
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Receptors, Opioid, delta
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Receptors, Opioid, mu
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Recombinant Fusion Proteins
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Ribosome Inactivating Proteins, Type 1
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dermorphin-saporin
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Substance P
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Freund's Adjuvant
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N-Glycosyl Hydrolases
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Saporins