Abstract
A murine monoclonal antibody (mAb), CLD3 (IgG(1),kappa), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tissues. By immunoprecipitation and using the matrix-assisted laser desorption/ionization-time of flight mass spectrometry approach, the antigenic specificity of CLD3 was determined to be heterodimeric Ku70 and Ku80 autoantigen, which was confirmed by Western blotting.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antibodies, Monoclonal / metabolism*
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Antibodies, Neoplasm / metabolism
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Antigens, Nuclear / immunology
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Antigens, Nuclear / metabolism*
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Binding Sites, Antibody
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Carcinoma, Hepatocellular / chemistry
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Carcinoma, Hepatocellular / immunology*
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DNA-Binding Proteins / immunology
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DNA-Binding Proteins / metabolism*
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Fluorescent Antibody Technique
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Humans
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Immunohistochemistry
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Ku Autoantigen
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Liver Neoplasms / immunology*
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Liver Neoplasms / metabolism
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Molecular Sequence Data
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Antibodies, Monoclonal
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Antibodies, Neoplasm
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Antigens, Nuclear
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DNA-Binding Proteins
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Xrcc6 protein, human
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Ku Autoantigen