Characterization of ionic currents in human mesenchymal stem cells from bone marrow

Stem Cells. 2005 Mar;23(3):371-82. doi: 10.1634/stemcells.2004-0213.

Abstract

This study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole-cell patch clamp and reverse transcription polymerase chain reaction (RT-PCR) techniques. Three types of outward currents were found in hMSCs, including a noise-like rapidly activating outward current inhibited by the large conductance Ca(2+)-activated K(+) channel (I(KCa)) blocker iberiotoxin, a transient outward K(+) current (I(to)) suppressed by 4-aminopyridine (4-AP), and a delayed rectifier K(+) current (IK(DR))-like ether-à-go-go (eag) K(+) channel. In addition, tetrodotoxin-sensitive sodium current (I(Na.TTX)) and nifedipine-sensitive L-type Ca(2+) current (I(Ca.L)) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT-PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for I(KCa), Kv4.2 and Kv1.4 for I(to), heag1 for IK(DR), hNE-Na for I(Na.TTX), and CACNAIC for I(Ca.L). These results demonstrate that multiple functional ion channel currents--that is, I(KCa), I(to), heag1, I(Na.TTX), and I(Ca.L)--are expressed in hMSCs from bone marrow.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Aminopyridine / pharmacology
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / drug effects
  • Bone Marrow Cells / physiology*
  • Calcium Channels, L-Type / drug effects
  • Calcium Channels, L-Type / genetics
  • Calcium Channels, L-Type / physiology
  • Cell Differentiation
  • Cells, Cultured
  • Ether-A-Go-Go Potassium Channels
  • Gene Expression / genetics
  • Humans
  • Ion Channels / genetics
  • Ion Channels / physiology*
  • Kv1.4 Potassium Channel
  • Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
  • Large-Conductance Calcium-Activated Potassium Channels
  • Membrane Potentials / drug effects
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / physiology*
  • NAV1.7 Voltage-Gated Sodium Channel
  • Nerve Tissue Proteins / genetics
  • Nifedipine / pharmacology
  • Patch-Clamp Techniques
  • Peptides / pharmacology
  • Potassium Channels / genetics
  • Potassium Channels, Calcium-Activated / drug effects
  • Potassium Channels, Calcium-Activated / genetics
  • Potassium Channels, Calcium-Activated / physiology
  • Potassium Channels, Voltage-Gated / drug effects
  • Potassium Channels, Voltage-Gated / genetics
  • Potassium Channels, Voltage-Gated / physiology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Shal Potassium Channels
  • Sodium Channels / drug effects
  • Sodium Channels / genetics
  • Sodium Channels / physiology
  • Tetrodotoxin / pharmacology

Substances

  • CACNA1C protein, human
  • Calcium Channels, L-Type
  • Ether-A-Go-Go Potassium Channels
  • Ion Channels
  • KCNA4 protein, human
  • KCND2 protein, human
  • KCNMA1 protein, human
  • Kv1.4 Potassium Channel
  • Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
  • Large-Conductance Calcium-Activated Potassium Channels
  • NAV1.7 Voltage-Gated Sodium Channel
  • Nerve Tissue Proteins
  • Peptides
  • Potassium Channels
  • Potassium Channels, Calcium-Activated
  • Potassium Channels, Voltage-Gated
  • RNA, Messenger
  • SCN9A protein, human
  • Shal Potassium Channels
  • Sodium Channels
  • Tetrodotoxin
  • iberiotoxin
  • 4-Aminopyridine
  • Nifedipine