Abstract
This study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole-cell patch clamp and reverse transcription polymerase chain reaction (RT-PCR) techniques. Three types of outward currents were found in hMSCs, including a noise-like rapidly activating outward current inhibited by the large conductance Ca(2+)-activated K(+) channel (I(KCa)) blocker iberiotoxin, a transient outward K(+) current (I(to)) suppressed by 4-aminopyridine (4-AP), and a delayed rectifier K(+) current (IK(DR))-like ether-à-go-go (eag) K(+) channel. In addition, tetrodotoxin-sensitive sodium current (I(Na.TTX)) and nifedipine-sensitive L-type Ca(2+) current (I(Ca.L)) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT-PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for I(KCa), Kv4.2 and Kv1.4 for I(to), heag1 for IK(DR), hNE-Na for I(Na.TTX), and CACNAIC for I(Ca.L). These results demonstrate that multiple functional ion channel currents--that is, I(KCa), I(to), heag1, I(Na.TTX), and I(Ca.L)--are expressed in hMSCs from bone marrow.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
4-Aminopyridine / pharmacology
-
Bone Marrow Cells / cytology
-
Bone Marrow Cells / drug effects
-
Bone Marrow Cells / physiology*
-
Calcium Channels, L-Type / drug effects
-
Calcium Channels, L-Type / genetics
-
Calcium Channels, L-Type / physiology
-
Cell Differentiation
-
Cells, Cultured
-
Ether-A-Go-Go Potassium Channels
-
Gene Expression / genetics
-
Humans
-
Ion Channels / genetics
-
Ion Channels / physiology*
-
Kv1.4 Potassium Channel
-
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
-
Large-Conductance Calcium-Activated Potassium Channels
-
Membrane Potentials / drug effects
-
Mesenchymal Stem Cells / cytology
-
Mesenchymal Stem Cells / drug effects
-
Mesenchymal Stem Cells / physiology*
-
NAV1.7 Voltage-Gated Sodium Channel
-
Nerve Tissue Proteins / genetics
-
Nifedipine / pharmacology
-
Patch-Clamp Techniques
-
Peptides / pharmacology
-
Potassium Channels / genetics
-
Potassium Channels, Calcium-Activated / drug effects
-
Potassium Channels, Calcium-Activated / genetics
-
Potassium Channels, Calcium-Activated / physiology
-
Potassium Channels, Voltage-Gated / drug effects
-
Potassium Channels, Voltage-Gated / genetics
-
Potassium Channels, Voltage-Gated / physiology
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Shal Potassium Channels
-
Sodium Channels / drug effects
-
Sodium Channels / genetics
-
Sodium Channels / physiology
-
Tetrodotoxin / pharmacology
Substances
-
CACNA1C protein, human
-
Calcium Channels, L-Type
-
Ether-A-Go-Go Potassium Channels
-
Ion Channels
-
KCNA4 protein, human
-
KCND2 protein, human
-
KCNMA1 protein, human
-
Kv1.4 Potassium Channel
-
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
-
Large-Conductance Calcium-Activated Potassium Channels
-
NAV1.7 Voltage-Gated Sodium Channel
-
Nerve Tissue Proteins
-
Peptides
-
Potassium Channels
-
Potassium Channels, Calcium-Activated
-
Potassium Channels, Voltage-Gated
-
RNA, Messenger
-
SCN9A protein, human
-
Shal Potassium Channels
-
Sodium Channels
-
Tetrodotoxin
-
iberiotoxin
-
4-Aminopyridine
-
Nifedipine