Arimidex inhibition on proliferation of human breast solid tumors measured by ATP bioluminescence

Edith Y T Tse; Wings T Y Loo; Mary N B Cheung; Louis W C Chow; Carter W Cheng

doi:10.1016/j.lfs.2004.09.016

Arimidex inhibition on proliferation of human breast solid tumors measured by ATP bioluminescence

Life Sci. 2004 Dec 31;76(7):827-34. doi: 10.1016/j.lfs.2004.09.016.

Authors

Edith Y T Tse¹, Wings T Y Loo, Mary N B Cheung, Louis W C Chow, Carter W Cheng

Affiliation

¹ Department of Surgery, University of Hong Kong Medical Centre, Pokfulam Road, Queen Mary Hospital, Hong Kong.

PMID: 15581914
DOI: 10.1016/j.lfs.2004.09.016

Abstract

To determine the degree of Arimidex (Anastrozole) inhibition on proliferation of human breast solid tumors in vitro by ATP bioluminescence assay. Breast cancer solid tumors with different hormone receptors and grading were collected from 38 Chinese women with invasive breast cancers. Tumors were treated with three concentrations of Arimidex (1.5 mM, 15 mM and 150 mM). ATP bioluminescence assay was used to measure the metabolic rate in order to determine the degree of inhibition of Arimidex on the breast cancer tumors by comparing to the untreated tumors. 15 mM Arimidex shows greatest inhibitory effect on the proliferation of solid tumors with ER-postive/PR-positive. It can also inhibit the growth of metastatic tumors and tumors with HER-2/neu expression. It shows greater inhibitory effect in lower grading of tumors then higher. Arimidex may effectively inhibit the growth of breast tumors in in vitro system by inhibiting aromatase and block estrogen dependent tumor growth.

MeSH terms

Adenosine Triphosphatases / analysis
Adenosine Triphosphatases / metabolism*
Adult
Aged
Aged, 80 and over
Anastrozole
Antineoplastic Agents, Hormonal / pharmacology*
Aromatase Inhibitors / pharmacology*
Breast Neoplasms / drug therapy*
Breast Neoplasms / enzymology
Breast Neoplasms / pathology
Carcinoma, Ductal, Breast / drug therapy*
Carcinoma, Ductal, Breast / enzymology
Carcinoma, Ductal, Breast / pathology
Cell Proliferation / drug effects
Female
Humans
Luminescent Measurements / methods
Luminescent Proteins / analysis
Luminescent Proteins / metabolism
Middle Aged
Nitriles / pharmacology*
Receptor, ErbB-2 / analysis
Receptor, ErbB-2 / metabolism
Receptors, Estrogen / analysis
Receptors, Estrogen / metabolism
Receptors, Progesterone / analysis
Receptors, Progesterone / metabolism
Triazoles / pharmacology*
Tumor Cells, Cultured

Substances

Antineoplastic Agents, Hormonal
Aromatase Inhibitors
Luminescent Proteins
Nitriles
Receptors, Estrogen
Receptors, Progesterone
Triazoles
Anastrozole
Receptor, ErbB-2
Adenosine Triphosphatases