Quantitative detection of methylated SOCS-1 , a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection

Biotechnol Lett. 2004 Aug;26(16):1289-93. doi: 10.1023/B:BILE.0000044922.43572.2d.

Abstract

Although methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Benzothiazoles
  • Biomarkers, Tumor / blood*
  • DNA Methylation
  • Diamines
  • Female
  • Genes, Tumor Suppressor
  • Genetic Predisposition to Disease / genetics
  • Genetic Testing / methods
  • Humans
  • Intracellular Signaling Peptides and Proteins / blood*
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Male
  • Methylation
  • Middle Aged
  • Organic Chemicals*
  • Polymerase Chain Reaction / methods*
  • Quinolines
  • Repressor Proteins / blood*
  • Repressor Proteins / metabolism*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Stomach Neoplasms / blood*
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / metabolism*
  • Suppressor of Cytokine Signaling 1 Protein
  • Suppressor of Cytokine Signaling Proteins

Substances

  • Benzothiazoles
  • Biomarkers, Tumor
  • Diamines
  • Intracellular Signaling Peptides and Proteins
  • Organic Chemicals
  • Quinolines
  • Repressor Proteins
  • SOCS1 protein, human
  • Suppressor of Cytokine Signaling 1 Protein
  • Suppressor of Cytokine Signaling Proteins
  • SYBR Green I