Abstract
Although methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Adult
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Benzothiazoles
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Biomarkers, Tumor / blood*
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DNA Methylation
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Diamines
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Female
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Genes, Tumor Suppressor
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Genetic Predisposition to Disease / genetics
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Genetic Testing / methods
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Humans
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Intracellular Signaling Peptides and Proteins / blood*
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Intracellular Signaling Peptides and Proteins / metabolism*
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Male
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Methylation
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Middle Aged
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Organic Chemicals*
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Polymerase Chain Reaction / methods*
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Quinolines
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Repressor Proteins / blood*
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Repressor Proteins / metabolism*
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Reproducibility of Results
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Sensitivity and Specificity
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Stomach Neoplasms / blood*
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Stomach Neoplasms / genetics
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Stomach Neoplasms / metabolism*
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Suppressor of Cytokine Signaling 1 Protein
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Suppressor of Cytokine Signaling Proteins
Substances
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Benzothiazoles
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Biomarkers, Tumor
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Diamines
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Intracellular Signaling Peptides and Proteins
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Organic Chemicals
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Quinolines
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Repressor Proteins
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SOCS1 protein, human
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Suppressor of Cytokine Signaling 1 Protein
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Suppressor of Cytokine Signaling Proteins
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SYBR Green I