Because there are no data available on the seroprevalence of hepatitis B in dental personnel in South Africa, a study was performed to assess the antibody status to hepatitis B surface antigen among 129 dental (preclinical) students and 62 dental hygienists at the Western Cape Dental School of South Africa. A total of 11 dental students and nine hygienists were positive to hepatitis B surface antibody, indicating a seroprevalence of 8.5% and 14.5%, respectively. These results, reported for the first time in dental personnel in South Africa, imply that dental students and hygienists in this part of the world are exposed to a significant risk of contracting hepatitis B.