A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor

AIDS. 2004 Aug 20;18(12):1729-32. doi: 10.1097/01.aids.0000131382.15232.dc.

Abstract

A Nepalese heterozygous carrier of a CCR5 mutant, designated 118delF, was characterized. There was a 3 basepair deletion at 352-354 in the CCR5 open reading frame, resulting in the deletion of the phe-118 residue located in the third transmembrane domain. The mutant protein has retained antigen specificity near the third extra-cellular loop (ECL3), but that of ECL2 is markedly reduced. The mutation has also abrogated HIV co-receptor activity. Clinically, the HIV disease had progressed slowly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • HIV Infections / genetics*
  • HIV Infections / immunology
  • HIV-1 / genetics*
  • HIV-1 / immunology
  • Heterozygote
  • Humans
  • Mutation / genetics
  • Mutation / immunology
  • RNA, Messenger / analysis
  • RNA, Viral / analysis
  • Receptors, CCR5 / genetics*
  • Receptors, CCR5 / immunology
  • Receptors, Chemokine / genetics
  • Receptors, Chemokine / immunology
  • Receptors, HIV / genetics*
  • Receptors, HIV / immunology
  • Sequence Deletion

Substances

  • RNA, Messenger
  • RNA, Viral
  • Receptors, CCR5
  • Receptors, Chemokine
  • Receptors, HIV