Abstract
Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Cell Culture Techniques / instrumentation
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Cell Culture Techniques / methods
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Cell Survival / physiology*
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Colony Count, Microbial / instrumentation
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Colony Count, Microbial / methods*
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Flow Cytometry / instrumentation
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Flow Cytometry / methods*
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Fluorescent Dyes
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Green Fluorescent Proteins
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Microscopy, Confocal / instrumentation
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Microscopy, Confocal / methods*
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods*
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Propidium
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Reagent Kits, Diagnostic*
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / isolation & purification
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Saccharomyces cerevisiae / physiology
Substances
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Fluorescent Dyes
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Reagent Kits, Diagnostic
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Green Fluorescent Proteins
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Propidium