Caveolin-1 gene is coordinately regulated with the multidrug resistance 1 gene in normal and leukemic bone marrow

Leuk Res. 2004 Sep;28(9):973-7. doi: 10.1016/j.leukres.2004.01.010.

Abstract

Caveolin-1 is a structural protein that may function as a scaffold for plasma membrane proteins, one of which is P-glycoprotein (P-gp), product of the multidrug resistance-1 (MDR-1) gene. We tested the hypothesis that if P-gp and caveolin-1 interacted physically, caveolin-1 and MDR-1 genes might be coordinately regulated; by quantifiying their gene expression with quantitative-polymerase chain reaction. MDR-1 and caveolin-1 gene expressions were normalized to an internal control and related to a fixed calibrator by a comparative cycle-threshold (CT) method. In four different groups of marrow samples (20 normal, 56 acute myeloid leukemias (AML) at diagnosis, 48 AMLs at relapse, and 51 regenerating marrows), caveolin-1 and MDR-1 gene expressions were positively correlated. In 65 samples with MDR-1 over-expression, caveolin-1 and MDR-1 expressions were also correlated. The coordinate expression of caveolin-1 and MDR-1 suggests that they may either interact physically, or are involved in the same aberrant pathway(s) activated during MDR-1 up-regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • Bone Marrow / pathology
  • Calibration
  • Caveolin 1
  • Caveolins / biosynthesis
  • Caveolins / genetics*
  • Disease Progression
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Leukemia, Myeloid / pathology*
  • Polymerase Chain Reaction / methods*
  • Reference Standards

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • CAV1 protein, human
  • Caveolin 1
  • Caveolins