[Effects of baicalin on the expression of pro-MMP-1 and MMP-3 in human gingival fibroblasts and periodontal ligament cells]

Zhonghua Kou Qiang Yi Xue Za Zhi. 2004 May;39(3):197-200.
[Article in Chinese]

Abstract

Objective: To investigate the influence of baicalin on the IL-1beta induced pro-MMP-1 in HGF and the effects of baicalin on MMP-3 expression in periodontal ligament cells (PDLCs).

Methods: The amount of secreted pro-MMP-1 and MMP-3 expression was detected by ELISA and cell immunochemistry.

Results: (1) The amount of secreted pro-MMP-1 (3.333 +/- 0.123) microg/L increased significantly following 1 microg/L of IL-1beta, compared with control group (1.960 +/- 0.180) microg/L. Addition of baicalin to cell culture medium for 1 hour following IL-1beta decreased pro-MMP-1 secretion in a dose-dependent manner in the range of 10 approximately 1,000 microg/L. (2) 1 microg/L IL-1beta could significantly stimulate the synthesis and secretion of MMP-3 in PDLCs. (3) The baicalin could not interfere the synthesis of MMP-3, but could inhibit the release of MMP-3 from PDLCs.

Conclusions: Baicalin could inhibit the secretion of pro-MMP-1 and MMP-3 expression in IL-1beta induced HGF and PDLCs, which suggests that baicalin may play an important role in preventing and treating periodontal disease.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Collagenases / biosynthesis*
  • Collagenases / genetics
  • Enzyme Precursors / biosynthesis*
  • Enzyme Precursors / genetics
  • Fibroblasts / enzymology
  • Fibroblasts / pathology
  • Flavonoids / pharmacology*
  • Gingiva / enzymology*
  • Gingiva / pathology
  • Humans
  • Interleukin-1 / pharmacology
  • Interleukin-1beta
  • Matrix Metalloproteinase 1
  • Metalloendopeptidases / biosynthesis*
  • Metalloendopeptidases / genetics
  • Peptide Fragments / pharmacology
  • Periodontal Ligament / enzymology*
  • Periodontal Ligament / pathology
  • Periodontitis / enzymology
  • Periodontitis / pathology
  • Scutellaria / chemistry

Substances

  • Enzyme Precursors
  • Flavonoids
  • Interleukin-1
  • Interleukin-1beta
  • Peptide Fragments
  • interleukin-1beta (163-171)
  • baicalin
  • Collagenases
  • Metalloendopeptidases
  • prostromelysin
  • Matrix Metalloproteinase 1