Abstract
In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.
Publication types
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
DNA, Ribosomal / analysis
-
Humans
-
Nasopharynx / virology
-
RNA, Ribosomal, 18S / genetics
-
RNA, Viral / analysis
-
RNA, Viral / isolation & purification
-
Reference Standards
-
Reverse Transcriptase Polymerase Chain Reaction / methods*
-
Sensitivity and Specificity
-
Severe Acute Respiratory Syndrome / diagnosis*
-
Severe Acute Respiratory Syndrome / virology
-
Severe acute respiratory syndrome-related coronavirus / genetics
-
Severe acute respiratory syndrome-related coronavirus / isolation & purification*
Substances
-
DNA, Ribosomal
-
RNA, Ribosomal, 18S
-
RNA, Viral