Application of preparative high-speed counter-current chromatography for separation of chlorogenic acid from Flos Lonicerae

J Chromatogr A. 2004 Feb 13;1026(1-2):185-90. doi: 10.1016/j.chroma.2003.11.004.

Abstract

Chlorogenic acid, an ester formed between caffeic acid and quinic acid, is a major phenolic compound in the traditional Chinese medicinal herb Flos Lonicerae. The separation and purification of chlorogenic acid from the crude extract of Flos Lonicerae was achieved by high-speed counter-current chromatography (HSCCC). A high acid, highly polar two-phase solvent system containing n-butanol-acetic acid-water (4:1:5) was run on a preparative scale. The upper phase was used as the mobile phase in the head to tail elution mode. A 300-mg quantity of the crude extract containing 5.97% chlorogenic acid was loaded on a 342-ml HSCCC column. Double separations were performed with the same solvent system yielding 16.9 mg chlorogenic acid at 94.8% purity with approximately 90% recovery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chlorogenic Acid / isolation & purification*
  • Countercurrent Distribution / methods*
  • Drugs, Chinese Herbal / chemistry*
  • Molecular Structure
  • Plants, Medicinal / chemistry*
  • Solvents

Substances

  • Drugs, Chinese Herbal
  • Solvents
  • Chlorogenic Acid