Detection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy material

J Virol Methods. 2004 Mar 1;116(1):79-88. doi: 10.1016/j.jviromet.2003.10.015.

Abstract

The latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-wild type LMP1 and compared the presence of the antibody staining with LMP1 DNA sequence analysis on clinical samples of nasopharyngeal carcinoma, peripheral T-cell lymphoma (PTCL), Hodgkin's disease, lymphoblastoid cell lines (LCL) from normal volunteers, and patients with nasopharyngeal carcinoma. The results demonstrate specificity of the monoclonal cocktail for detecting the non-wild type LMP1 and the ability to sub-differentiate between the mediterranean type of LMP1 and the CAO-LMP1. Double immunofluorescence on paraffin material using the traditional CS1-4 monoclonal antibodies and the CAO-cocktail revealed no dual population of cells in the biopsy material from the Asian region.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal* / immunology
  • Antibodies, Viral / immunology
  • Antibody Specificity
  • Antigens, Viral / immunology
  • Biopsy
  • Cell Line, Transformed
  • Cell Line, Tumor
  • DNA, Viral / analysis
  • DNA, Viral / isolation & purification
  • Epstein-Barr Virus Infections / virology
  • Genes, Viral
  • Hodgkin Disease / virology*
  • Humans
  • Immunohistochemistry
  • Lymphocytes / virology*
  • Lymphoma, T-Cell, Peripheral / virology*
  • Molecular Sequence Data
  • Nasopharyngeal Neoplasms / virology*
  • Nucleic Acid Amplification Techniques
  • Oncogene Proteins, Viral / analysis
  • Oncogene Proteins, Viral / chemistry
  • Oncogene Proteins, Viral / genetics
  • Oncogene Proteins, Viral / immunology
  • Sequence Deletion
  • Viral Matrix Proteins / analysis*
  • Viral Matrix Proteins / chemistry
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Viral
  • DNA, Viral
  • EBV-associated membrane antigen, Epstein-Barr virus
  • Oncogene Proteins, Viral
  • Viral Matrix Proteins