Abstract
The latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-wild type LMP1 and compared the presence of the antibody staining with LMP1 DNA sequence analysis on clinical samples of nasopharyngeal carcinoma, peripheral T-cell lymphoma (PTCL), Hodgkin's disease, lymphoblastoid cell lines (LCL) from normal volunteers, and patients with nasopharyngeal carcinoma. The results demonstrate specificity of the monoclonal cocktail for detecting the non-wild type LMP1 and the ability to sub-differentiate between the mediterranean type of LMP1 and the CAO-LMP1. Double immunofluorescence on paraffin material using the traditional CS1-4 monoclonal antibodies and the CAO-cocktail revealed no dual population of cells in the biopsy material from the Asian region.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antibodies, Monoclonal* / immunology
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Antibodies, Viral / immunology
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Antibody Specificity
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Antigens, Viral / immunology
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Biopsy
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Cell Line, Transformed
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Cell Line, Tumor
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DNA, Viral / analysis
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DNA, Viral / isolation & purification
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Epstein-Barr Virus Infections / virology
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Genes, Viral
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Hodgkin Disease / virology*
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Humans
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Immunohistochemistry
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Lymphocytes / virology*
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Lymphoma, T-Cell, Peripheral / virology*
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Molecular Sequence Data
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Nasopharyngeal Neoplasms / virology*
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Nucleic Acid Amplification Techniques
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Oncogene Proteins, Viral / analysis
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Oncogene Proteins, Viral / chemistry
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Oncogene Proteins, Viral / genetics
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Oncogene Proteins, Viral / immunology
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Sequence Deletion
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Viral Matrix Proteins / analysis*
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Viral Matrix Proteins / chemistry
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Viral Matrix Proteins / genetics
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Viral Matrix Proteins / immunology
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Antigens, Viral
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DNA, Viral
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EBV-associated membrane antigen, Epstein-Barr virus
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Oncogene Proteins, Viral
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Viral Matrix Proteins