Application of high-speed counter-current chromatography to the preparative separation and purification of baicalin from the Chinese medicinal plant Scutellaria baicalensis

J Chromatogr A. 2003 Oct 31;1017(1-2):117-23. doi: 10.1016/j.chroma.2003.08.012.

Abstract

Baicalin was separated and purified for the first time from the traditional Chinese medicinal plant Scutellaria baicalensis Georgi by high-speed counter-current chromatography. Crude baicalin was obtained by extraction with methanol-water (70:30, v/v) from S. baicalensis Georgi. The separation was performed in two steps with a two-phase solvent system composed of n-butanol-water (1:1, v/v), in which the lower phase was used as the mobile phase at a flow-rate of 1.0 ml min(-1) in the head-to-tail elution mode. A total of 37.0 mg of baicalin at 96.5% purity was yielded from 200 mg of the crude baicalin (containing 21.6% baicalin) with 86.0% recovery as determined by HPLC analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Countercurrent Distribution / methods*
  • Flavonoids / isolation & purification*
  • Plants, Medicinal / chemistry*
  • Scutellaria baicalensis / chemistry*

Substances

  • Flavonoids
  • baicalin