Abstract
We have constructed recombinant baculoviruses and vaccinia viruses containing cloned DNA, encoding either the envelope protein alone or all of the structural proteins (core, membrane and envelope) of louping ill virus. Glycosylated viral envelope protein, presented both inside and on the surface of insect and mammalian cells, was expressed by all four recombinant viruses. Differences in antigenic presentation of the envelope protein were observed between the envelope protein and structural protein constructs as well as between the insect and mammalian cell expression systems. Despite the expression of epitopes known to elicit neutralizing and protective antibodies when present in authentic antigen, the recombinant envelope protein expressed by either vector failed to induce, in mice or rabbits, either neutralizing or protective antibodies against louping ill virus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Viral / biosynthesis*
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Baculoviridae / genetics
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Baculoviridae / immunology*
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Base Sequence
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Encephalitis Viruses, Tick-Borne / genetics
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Encephalitis Viruses, Tick-Borne / growth & development
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Encephalitis Viruses, Tick-Borne / immunology*
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Genes, Viral
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Genetic Vectors
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Immune Sera / chemistry
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Immunization, Passive
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Louping Ill / immunology
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Mice
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Molecular Sequence Data
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Moths / genetics
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Neutralization Tests
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Rabbits
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Recombinant Proteins / immunology
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Togaviridae Infections / immunology
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Tunicamycin / pharmacology
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Vaccinia virus / genetics
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Vaccinia virus / immunology*
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Viral Envelope Proteins / biosynthesis
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Viral Envelope Proteins / immunology*
Substances
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Antibodies, Viral
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Immune Sera
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Recombinant Proteins
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Viral Envelope Proteins
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Tunicamycin
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NEG envelope glycoprotein, Negishi virus