Guided tissue regeneration using a polylactic acid barrier. Part I: Environmental effects on bacterial colonization

J Clin Periodontol. 2003 Jan;30(1):19-25. doi: 10.1034/j.1600-051x.2003.300104.x.

Abstract

Objectives: The purpose of this study was to assess the dynamics of bacterial colonization in intra-osseous defects following guided tissue regeneration (GTR) therapy using a resorbable barrier.

Patients and methods: In each of 30 patients, one intra-osseous defect was treated with GTR using a polylactic acid membrane (Guidor). Plaque samples were taken from the defect site, other teeth and mucous membranes following initial therapy (baseline), and at 3, 6 and 12 months after periodontal surgery. Additionally, samples were taken from the defect sites at 1, 2 and 4 weeks. Actinobacillus actinomycetemcomitans (A.a.), Porphyromonas gingivalis (P.g.), and Bacteroides forsythus (B.f.) were detected by polymerase chain reaction (PCR). Supportive periodontal therapy was performed at 3-month intervals.

Results: In the 29 patients completing the study, the assessed microflora was detected in 3 (A.a.), 13 (P.g.) and 14 (B.f.) defect sites at baseline, in 2 (A.a.), 2 (P.g.) and 2 (B.f.) following surgical debridement, and in 6 (A.a.), 10 (P.g.) and 22 (B.f.) at 12 months. Defect site colonization following GTR therapy was significantly correlated with presurgical colonization at other assessed teeth (A.a. and P.g.: tau = 0.45 and 0.66, respectively; P < 0.001), or on mucous membranes (B.f.: tau = 0.44, P < 0.001).

Conclusion: The colonization of periodontal pathogens at sites treated by GTR may correlate with the intra-oral presence of these pathogens before surgery. If colonization of GTR sites by periodontal pathogens is to be prevented, intra-oral suppression/eradication of these pathogens may be required before surgery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorbable Implants*
  • Adolescent
  • Adult
  • Aged
  • Aggregatibacter actinomycetemcomitans / growth & development
  • Alveolar Bone Loss / microbiology
  • Alveolar Bone Loss / surgery
  • Bacteroides / growth & development
  • Biocompatible Materials
  • Citrates
  • Dental Plaque / microbiology
  • Female
  • Follow-Up Studies
  • Gram-Negative Bacteria / physiology*
  • Guided Tissue Regeneration, Periodontal / instrumentation*
  • Guided Tissue Regeneration, Periodontal / methods
  • Humans
  • Lactic Acid*
  • Male
  • Membranes, Artificial*
  • Middle Aged
  • Mouth / microbiology*
  • Mouth Mucosa / microbiology
  • Periodontitis / microbiology
  • Periodontitis / surgery
  • Polyesters
  • Polymers*
  • Porphyromonas gingivalis / growth & development
  • Statistics as Topic
  • Tooth / microbiology

Substances

  • Biocompatible Materials
  • Citrates
  • Guidor
  • Membranes, Artificial
  • Polyesters
  • Polymers
  • Lactic Acid
  • poly(lactide)