Abstract
The in vivo host response to two gelatin-based hydrogel systems of varying crosslinking modalities and loaded with the anti-inflammatory agent dexamethasone sodium phosphate was investigated. Either gelatin was chemically crosslinked with glutaraldehyde, or polyethyleneglycol diacrylate was photopolymerized around gelatin to form interpenetrating networks. The subcutaneous cage implant system was utilized to determine differential leukocyte concentrations in the inflammatory exudate surrounding the materials as indices for biocompatibility and drug efficacy in vivo. Most of the crosslinked gelatin-based materials, either via glutaraldehyde fixation or interpenetrating network formation, elicited stronger inflammatory responses than either of the starting materials, gelatin and polyethyleneglycol diacrylate. In general, dexamethasone delayed and intensified the inflammatory response. The loss of material mass did not correlate directly with the degree of cellular inflammatory response, but increased with longer implantation time and decreased with more extensive fixation.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Biocompatible Materials / chemical synthesis
-
Biocompatible Materials / chemistry
-
Biocompatible Materials / pharmacokinetics
-
Biocompatible Materials / pharmacology*
-
Cross-Linking Reagents / chemistry
-
Cross-Linking Reagents / pharmacokinetics
-
Cross-Linking Reagents / pharmacology
-
Dexamethasone / administration & dosage
-
Dexamethasone / analogs & derivatives*
-
Dexamethasone / immunology
-
Dexamethasone / pharmacokinetics
-
Drug Carriers / chemistry*
-
Drug Carriers / pharmacokinetics
-
Drug Carriers / pharmacology*
-
Exudates and Transudates / immunology
-
Exudates and Transudates / metabolism
-
Female
-
Gelatin / chemistry*
-
Gelatin / pharmacokinetics
-
Gelatin / pharmacology*
-
Glutaral / chemistry
-
Glutaral / pharmacokinetics
-
Hydrogels / chemistry*
-
Hydrogels / pharmacokinetics
-
Hydrogels / pharmacology*
-
Neutrophils / drug effects
-
Neutrophils / immunology
-
Neutrophils / metabolism
-
Photochemistry
-
Polyethylene Glycols / chemistry
-
Polyethylene Glycols / pharmacokinetics
-
Rats
-
Rats, Sprague-Dawley
Substances
-
Biocompatible Materials
-
Cross-Linking Reagents
-
Drug Carriers
-
Hydrogels
-
dexamethasone 21-phosphate
-
Polyethylene Glycols
-
Dexamethasone
-
Gelatin
-
Glutaral