A prospective case control study of the association of Gianotti-Crosti syndrome with human herpesvirus 6 and human herpesvirus 7 infections

Pediatr Dermatol. 2002 Nov-Dec;19(6):492-7. doi: 10.1046/j.1525-1470.2002.00217.x.

Abstract

Gianotti-Crosti syndrome (GCS) is known to be associated with hepatitis B and Epstein-Barr virus (EBV) infections. Apart from a single case report based on serology alone, there are no published data on an association between GCS and human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) primary infections or reactivations. Our aim was to investigate the association between GCS and HHV-6 and HHV-7 infections. Ten patients diagnosed with GCS at a primary care practice over an 18-month period were recruited. Controls were age- and sex-matched patients with unrelated symptoms requiring venepuncture for other indications. Blood specimens were collected from patients and controls at presentation, and from patients 4 weeks later. Virologic evidence of HHV-6 and HHV-7 infection was sought in peripheral blood leukocytes and plasma using polymerase chain reaction (PCR) for viral DNA, reverse transcriptase polymerase chain reaction (RT-PCR) for HHV-6 U91 mRNA transcripts, and serology. Serology for EBV and hepatitis B virus was done. In contrast to the 10 controls, 2 patients (both infants) with clinically diagnosed GCS had evidence of active HHV-6 infection. This was demonstrated by detection of viral DNA in the absence of antibody in the acute plasma specimens and HHV-6 DNA viral loads of more than 5.3 log10 genome copies/5 microl in the whole blood specimens, a profile previously shown to be diagnostic of recent primary HHV-6 infection. None of the patients had evidence of recent EBV or hepatitis B infection. We conclude that primary HHV-6 infection may be associated with GCS in some infants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrodermatitis / virology*
  • Adolescent
  • Adult
  • Case-Control Studies
  • Child
  • Child, Preschool
  • DNA, Viral / blood
  • Enzyme-Linked Immunosorbent Assay
  • Exanthema Subitum / complications*
  • Female
  • Herpesvirus 6, Human / isolation & purification*
  • Herpesvirus 7, Human / isolation & purification*
  • Humans
  • Infant
  • Male
  • Prospective Studies
  • Reverse Transcriptase Polymerase Chain Reaction
  • Roseolovirus Infections / complications*
  • Statistics, Nonparametric

Substances

  • DNA, Viral