New variations in intron 4 of growth hormone gene in Chinese native chickens

J Hered. 2002 Jul-Aug;93(4):277-9. doi: 10.1093/jhered/93.4.277.

Abstract

Polymorphism in intron 4 of chicken growth hormone (cGH) gene was studied in 20 Chinese native chicken populations and broiler or layer populations. A total of eight restriction digestion profiles were identified in intron 4 and confirmed by sequencing. Among 20 populations, there were distinctively different allele numbers and frequencies of intron 4 restriction fragment length polymorphisms (RFLPs) between Chinese native chickens and broilers or layers. Two new alleles, allele D and allele E, were identified in Taihe Silkies. Allele D was also identified in other Chinese native breeds and a 50 bp fragment deletion was identified in allele E.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chickens / genetics*
  • DNA Primers
  • Growth Hormone / genetics*
  • Introns
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Sequence Analysis, DNA

Substances

  • DNA Primers
  • Growth Hormone