Objective: To observe the effect of rat serum containing Buyanghuanwu decoction (BYHWT) on the proliferation of cultured rat cortical neurons, so as to understand the mechanism of BYHWT in the treatment of hypoxia brain damage.
Methods: The growth of cultured rat cortical neurons were observed by MTT assay to evaluate the effect of the serum containing BYHWT on the neurons cultured in both normal and hypoxia conditions.
Results: BYHWT significantly promoted proliferation of the neurons cultured under both normal and hypoxia conditions, in comparison with the response of the cells to drug-free serum (P<0.05).
Conclusion: Some of the constituents of BYHWT in rat serum can promote the proliferation of rat cortical neurons cultured in both normal and hypoxia conditions.