Post-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA

Nucleic Acids Res. 2002 Apr 1;30(7):1664-9. doi: 10.1093/nar/30.7.1664.

Abstract

Double-stranded RNA (dsRNA) induces gene-specific silencing in organisms from fungi to animals, a phenomenon known as RNA interference (RNAi). RNAi represents an evolutionarily conserved system to protect against aberrant expression of genes and a powerful tool for gene manipulation. Despite reports that RNAi can be induced in vertebrates, severe sequence-non-specific effects of long dsRNA have been documented in various systems. It has recently been shown in cultured mammalian cells that small interfering RNAs (siRNAs) of 21-23 nt can mediate RNAi but bypass the non-specific response induced by longer dsRNAs. However, the effectiveness of siRNAs has not been demonstrated in living vertebrates. In addition, the mechanism of siRNA suppression of gene expression in vertebrate cells remains to be elucidated. Here we show that synthetic 21 nt siRNAs can specifically inhibit the expression of exogenously introduced as well as endogenous genes in the embryos of Xenopus laevis. siRNAs significantly reduced the steady-state amount of both the mRNA and protein of the cognate gene target. Moreover, co-injection of siRNA with the target RNA transcript specifically suppressed the activity of the latter. Taken together, our findings establish siRNA-mediated post-transcriptional suppression of gene expression in Xenopus embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Embryo, Nonmammalian / drug effects*
  • Embryo, Nonmammalian / metabolism
  • Gene Expression Regulation / drug effects
  • Luciferases / genetics
  • Luciferases / metabolism
  • Oligoribonucleotides / administration & dosage*
  • RNA, Double-Stranded / administration & dosage*
  • Recombinant Fusion Proteins / administration & dosage
  • Recombinant Fusion Proteins / drug effects
  • Recombinant Fusion Proteins / metabolism
  • Transcription, Genetic
  • Xenopus laevis

Substances

  • Oligoribonucleotides
  • RNA, Double-Stranded
  • Recombinant Fusion Proteins
  • Luciferases