[Detection of Immunoglobulin light chain restriction of mucosa-associated lymphoid tissue type gastric lymphoma using mRNA in situ hybridization]

Zhonghua Bing Li Xue Za Zhi. 2001 Feb;30(1):31-4.
[Article in Chinese]

Abstract

Objective: To evaluate the usefulness of in situ hybridization for Immunoglobulin kappa and lambda light chain mRNA to detect light chain restriction in the diagnosis of primary gastric lymphoma (PGL) of mucosa-associated lymphoid tissue (MALT) type.

Methods: Twenty-seven cases of PGL of MALT type and 5 cases of gastritis were analyzed using in situ hybridization with fluorescent isothiocyanate (FITC)-labeled oligonucleotide probes for kappa and lambda light chain mRNA. The kappa:lambda ratios of tumor cells, lymphocytes and plasma cells were analyzed to detect light chain restriction and clonal plasma cells.

Results: Light chain restriction was found in 10 of 27 (37%) cases of PGL cases (in 5/9 low grade and 5/18 in high grade). Clonal plasma cells were detected in low grade but not in high-grade cases. No light chain restriction was found in gastritis specimens, the adjacent tissue of tumors or the distant stomach mucosa in lymphoma cases.

Conclusions: The detection of clonality using mRNA in situ hybridization could be a helpful diagnostic tool for gastric lymphoma. The presence of clonal plasma cells can be a marker of the early lesion of low-grade PGL.

Publication types

  • English Abstract

MeSH terms

  • Diagnosis, Differential
  • Gastric Mucosa / chemistry
  • Gastritis / metabolism
  • Humans
  • Immunoglobulin kappa-Chains / analysis*
  • Immunoglobulin kappa-Chains / genetics
  • Immunoglobulin lambda-Chains / analysis*
  • Immunoglobulin lambda-Chains / genetics
  • In Situ Hybridization
  • Lymphoma, B-Cell, Marginal Zone / chemistry*
  • Plasma Cells / pathology
  • RNA, Messenger / analysis

Substances

  • Immunoglobulin kappa-Chains
  • Immunoglobulin lambda-Chains
  • RNA, Messenger