Determination of vitamin B12 in pharmaceutical preparations by a highly sensitive fluorimetric method

Fresenius J Anal Chem. 2000 Dec;368(8):836-8. doi: 10.1007/s002160000595.

Abstract

A fluorimetric method for the determination of vitamin B12 has been developed. The fluorescence emission was measured at lambda(ex)/lambda(em)275/305 nm in phosphate buffer solution (pH 7.0), and the experimental variables and possible interference were studied. The linear calibration range was 1.000 ng/mL to 100.0 ng/mL with a correlation coefficient of 0.9994 and a detection limit of 0.1 ng/mL. The method is rapid, simple and highly sensitive. It was used to determine vitamin B12 in pharmaceutical preparations. The recovery was 96%-98% and the relative standard deviation was in the range of 1.8%-2.7%. The results agreed with those obtained by spectrophotometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Culture Media
  • Drug Contamination
  • Eukaryota / chemistry
  • Hydrogen-Ion Concentration
  • Indicators and Reagents
  • Solutions
  • Spectrometry, Fluorescence
  • Tablets
  • Vitamin B 12 / analysis*

Substances

  • Culture Media
  • Indicators and Reagents
  • Solutions
  • Tablets
  • Vitamin B 12