Preparative isolation and purification of lutein from the microalga chlorella vulgaris by high-speed counter-current chromatography

H B Li; F Chen; T Y Zhang; F Q Yang; G Q Xu

doi:10.1016/s0021-9673(00)00987-0

Preparative isolation and purification of lutein from the microalga chlorella vulgaris by high-speed counter-current chromatography

J Chromatogr A. 2001 Jan 5;905(1-2):151-5. doi: 10.1016/s0021-9673(00)00987-0.

Authors

H B Li¹, F Chen, T Y Zhang, F Q Yang, G Q Xu

Affiliation

¹ Department of Botany, The University of Hong Kong, China.

PMID: 11206781
DOI: 10.1016/s0021-9673(00)00987-0

Abstract

High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane-ethanol-water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chlorella / chemistry*
Chromatography, High Pressure Liquid / methods*
Lutein / isolation & purification*
Spectrophotometry, Ultraviolet

Substances

Lutein