Differential gene expression in nasopharyngeal carcinoma cells

Life Sci. 2000 Jul 14;67(8):923-36. doi: 10.1016/s0024-3205(00)00684-6.

Abstract

Nasopharyngeal carcinoma (NPC) is a common cancer among southern Chinese. The profile of gene expression in NPC cells is largely unknown. In this study, we have examined differential gene expression in non-malignant and malignant nasopharyngeal epithelial (NPE) cells using a cDNA array hybridization method. A total of 42 genes were identified to be expressed in either non-malignant and malignant NPE cells or both. Thirteen of these genes were overexpressed in malignant NPE cells. These includes nuclear factor (NF90), FOS-related antigen 1 (FRA- 1), cytoplasmic dynein light chain (HDLC1), replication factor C (RFC1), nucleoside diphosphate kinase B, UV excision repair protein (RAD23A), insulin-like growth factor receptor II, transcription initiation factor TFIID subunit (TAFII31), growth factor receptor-bound protein 2 (GRB2), UV excision repair protein (RAD23B), glutathione peroxidase, Y box binding protein 1 and heat shock protein 86. In contrast, expression of nine genes was suppressed in malignant NPE cells. These includes calgranulin A, calgranulin B, neutrophil activating protein (ENA-78), heat shock protein 27, integrin beta-1, integrin beta-4, cyclin-dependent kinase inhibitor 1A (p21), interleukin-8 and tyrosine protein kinase receptor (RET). Differential expression of calgranulin A, calgraunlin B, ENA-78, FRA-1 and NF90 in non-malignant and malignant nasopharyngeal epithelial cells was confirmed by RT-PCR analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium-Binding Proteins / genetics
  • Calgranulin A
  • Calgranulin B
  • Chemokine CXCL5
  • Chemokines, CXC*
  • DNA, Complementary / analysis
  • DNA-Binding Proteins / genetics
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Interleukin-8 / analogs & derivatives
  • Interleukin-8 / genetics
  • NFATC Transcription Factors
  • Nasopharyngeal Neoplasms / genetics*
  • Nasopharynx / metabolism
  • Nuclear Factor 90 Proteins
  • Nuclear Proteins*
  • Proto-Oncogene Proteins c-fos / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors / genetics
  • Tumor Cells, Cultured

Substances

  • CXCL5 protein, human
  • Calcium-Binding Proteins
  • Calgranulin A
  • Calgranulin B
  • Chemokine CXCL5
  • Chemokines, CXC
  • DNA, Complementary
  • DNA-Binding Proteins
  • Interleukin-8
  • NFATC Transcription Factors
  • Nuclear Factor 90 Proteins
  • Nuclear Proteins
  • Proto-Oncogene Proteins c-fos
  • Transcription Factors
  • fos-related antigen 1