Abstract
Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Caco-2 Cells
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Cell Line
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Cell Polarity*
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Dogs
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Epithelial Cells / virology
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Gallbladder / cytology
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Gene Transfer Techniques*
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Genetic Vectors / genetics*
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Humans
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Intestinal Mucosa / virology*
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Lentivirus / genetics*
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Membrane Glycoproteins*
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Mice
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Moloney murine leukemia virus / genetics
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Transduction, Genetic
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Viral Envelope Proteins / genetics
Substances
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G protein, vesicular stomatitis virus
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Membrane Glycoproteins
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Viral Envelope Proteins