Morphine and methadone have different effects on calcium channel currents in neuroblastoma cells

Brain Res. 2000 Jul 7;870(1-2):199-203. doi: 10.1016/s0006-8993(00)02369-6.

Abstract

Using patch-clamp technique, cellular calcium channel currents were studied on nine mouse neuroblastoma N1E115 cells. Both morphine and methadone decreased the T-type calcium currents in dose-dependent fashion. These effects were partially blocked by naloxane. On L-type calcium currents, morphine showed no effect. However, methadone inhibited the L-calcium currents in dose-dependent fashion. This effect was not antagonised by naloxone. Hence, the action of methadone on L-calcium channel is probably not associated with mu receptors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium Channels / physiology*
  • Membrane Potentials / drug effects
  • Membrane Potentials / physiology
  • Methadone / pharmacology*
  • Mice
  • Morphine / pharmacology*
  • Naloxone / pharmacology
  • Narcotic Antagonists / pharmacology
  • Narcotics / pharmacology*
  • Neuroblastoma
  • Neurons / drug effects
  • Neurons / physiology*
  • Patch-Clamp Techniques
  • Tumor Cells, Cultured

Substances

  • Calcium Channels
  • Narcotic Antagonists
  • Narcotics
  • Naloxone
  • Morphine
  • Methadone