Sequencing of argentinated peptides by means of electrospray tandem mass spectrometry

Anal Chem. 1999 Jul 1;71(13):2364-72. doi: 10.1021/ac981351n.

Abstract

A strategy for semiautomatic sequencing of argentinated (silver-containing) oligopeptides has been developed. Sequencing is based on a search algorithm that identifies a triplet peak relationship in a product ion spectrum of the [M + Ag]+ ion of an oligopeptide. The ions that constitute a triplet are [bn + OH + Ag]+, [bn - H + Ag]+, and [a(n) - H + Ag]+, which are separated by 18 and 28 m/z units, respectively. The difference in the m/z values of adjacent triplets identifies the residue that is "cleaved". Observation of the [yn + H + Ag]+ ion containing the cleaved residue confirms the assignment. Sequencing of argentinated tryptic peptides may prove useful for automated proteome analysis via the sequence tag method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Hydrolysis
  • Mass Spectrometry
  • Molecular Sequence Data
  • Oligopeptides / analysis*
  • Oligopeptides / chemistry
  • Silver / chemistry*
  • Trypsin

Substances

  • Oligopeptides
  • Silver
  • Trypsin