GEO help: Mouse over screen elements for information.
Status
Public on Jul 08, 2005
Title
Day6 Genistein treatment replicate 2
Sample type
RNA
Source name
Thymuse
Organism
Mus musculus Characteristics
Femal C57BL/6, ovariectomized
Extracted molecule
total RNA
Label
biotin
Description
21-day-old felmale C57BL/6 mice from Harlan (Indianapolis, IN) were ovariectomized on day 26 and fed with phytoestrogen-free diet two days later (AIN-93G purified rodent diet, Dyets, In., Bethlehem, PA). Experimental treatments began on day 30. Animals were fed with a diet containing 1500 ppm genistein (Indofine Chemicals, Somerville, NJ) ad libitum by adding genistein to the AIN-93G diet. RNA were extracted at day 6 after experiment started. Total 6 animals were treated. RNA samples were pooled from 3 animals, resulting two independent biological replicates.
Data processing
Affymetrix Microarray Suite Version 5.0
Submission date
Jul 07, 2005
Last update date
Jul 07, 2005
Contact name
Lei Liu
E-mail(s)
leiliu@uiuc.edu Phone
217-265-5061
Organization name
University of Illinois at Urbana-Champaign
Department
W. M. Keck Center
Street address
1201 W. Gregory
City
Urbana
State/province
IL
ZIP/Postal code
61801
Country
USA
Platform ID
GPL339 Series (1)
GSE2889 Gene Expression Profiling of 17 beta-Estradiol and Genistein Effects on Mouse Thymus
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript wa present (P), absent (A), marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
99.5
P
0.020022
AFFX-BioB-M_at
86.2
P
0.000225
AFFX-BioB-3_at
52.8
P
0.012547
AFFX-BioC-5_at
168.1
P
0.000581
AFFX-BioC-3_at
85.7
P
0.000258
AFFX-BioDn-5_at
170
P
0.00011
AFFX-BioDn-3_at
890
P
0.000258
AFFX-CreX-5_at
1515.3
P
0.000044
AFFX-CreX-3_at
2362.8
P
0.000044
AFFX-DapX-5_at
11.5
A
0.139482
AFFX-DapX-M_at
18.3
A
0.195266
AFFX-DapX-3_at
2.1
A
0.969024
AFFX-LysX-5_at
1.8
A
0.712257
AFFX-LysX-M_at
4.4
A
0.712257
AFFX-LysX-3_at
23.1
P
0.006871
AFFX-PheX-5_at
1.1
A
0.932322
AFFX-PheX-M_at
3.7
A
0.843268
AFFX-PheX-3_at
5.7
A
0.737173
AFFX-ThrX-5_at
3
A
0.932322
AFFX-ThrX-M_at
2.1
A
0.659339
22690 610 Kbytes .Supplementary data files not provided
