|
| Status |
Public on Aug 31, 2009 |
| Title |
activin-treated ES cells #1 |
| Sample type |
RNA |
| |
|
| Source name |
ES cells in defined media + activin 10ng/ml, 2 hrs
|
| Organism |
Mus musculus |
| Characteristics |
cell line: E14Tg2a
cell type: mouse embryonic stem cells
developmental stage: E14
condition: activin
|
| Treatment protocol |
Untreated, 10ng/ml activin for 2 hrs, or 5uM SB431542 for 24 hrs.
|
| Growth protocol |
Defined ES cell media (N2B27) supplemented with BMP4 (20ng/ml) and LIF (10^3U/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy RNA purification.
|
| Label |
biotin
|
| Label protocol |
Oligo-dT first-strand synthesis, biotin labelled via in vitro transcription.
|
| |
|
| Hybridization protocol |
Per standard Affymetrix protocol.
|
| Scan protocol |
Automated process conducted using the Agilent GeneArray Scanner.
|
| Description |
activin 4
|
| Data processing |
Bioconductor package in R; RMA normalization.
|
| |
|
| Submission date |
Aug 30, 2009 |
| Last update date |
Aug 30, 2009 |
| Contact name |
Jay Vivian |
| E-mail(s) |
jvivian@kumc.edu
|
| Phone |
(913) 588-0341
|
| Fax |
(913) 588-8287
|
| Organization name |
University of Kansas Medical Center
|
| Department |
Pathology
|
| Street address |
3901 Rainbow Blvd.
|
| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE17879 |
Activin/Nodal signaling in mouse embryonic stem cells |
|
