|
| Status |
Public on Sep 07, 2008 |
| Title |
A Functional Survey of Light-Responsive Genes Using A Rice NSF45K Microarray |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
|
| Summary |
We performed expression profiling experiments on two-week old rice leaf tissues grown in light and dark condition with the rice 47k array. Four biological replicates and a dye-swap were used. To generate more consistent results on light vs dark response in rice, biological replicates were prepared from 4 different varieties such as Kitaake, Nipponbare, Tapei309 and IR24 because individual genetic background can affect to expression profile (Townsend et al., 2003). Gene expression was assayed by probing the microarray with pooled Cy3 and Cy5 labeled cDNAs derived from 0.5-1.0ug high quality mRNA prepared from leaf samples.
Keywords: Direct comparison
|
| |
|
| Overall design |
16 hybs total
|
| Web link |
http://www.ricearray.org
|
| |
|
| Contributor(s) |
Jung KH |
| Citation(s) |
18836531, 18725934 |
| |
| Submission date |
Jun 22, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Jia Liu |
| E-mail(s) |
jliu@jcvi.org
|
| URL |
http://www.tigr.org/tdb/potato
|
| Organization name |
Plant Genomics
|
| Street address |
9712 Medical Center Drive
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL4105 |
NSF Rice Oligonucleotide Array 45K (23K A) |
| GPL4106 |
NSF Rice Oligonucleotide Array 45K (23K B) |
|
| Samples (16)
|
| GSM205253 |
13360999_532nm_720PMT_635nm_800PMT_0045b.gpr |
| GSM205254 |
13371000_532nm_720PMT_635nm_800PMT_45kb.gpr |
| GSM205255 |
13371001_532nm_650PMT_635nm_840PMT_45Kb.gpr |
| GSM205256 |
13371002_532nm_660PMT_635nm_840PMT_45Kb.gpr |
| GSM205257 |
13371003_532nm_660PMT_635nm_840PMT_45Kb.gpr |
| GSM205258 |
13371004_532nm_660PMT_635nm_840PMT_45Kb.gpr |
| GSM205259 |
13371005_532nm_640PMT_635nm_840PMT_45Kb.gpr |
| GSM205260 |
13371006_532nm_660PMT_635nm_840PMT_45Kb.gpr |
| GSM205261 |
13371166_532nm_660PMT_635nm_840PMT_45Ka.gpr |
| GSM205262 |
13371168_532nm_660PMT_635nm_840PMT_45Ka.gpr |
| GSM205263 |
13371169_532nm_660PMT_635nm_840PMT_45Ka.gpr |
| GSM205264 |
13371170_532nm_650PMT_635nm_840PMT_45Ka.gpr |
| GSM205265 |
13371171_532nm_660PMT_635nm_840PMT_45Ka.gpr |
| GSM205266 |
13371172_532nm_660PMT_635nm_840PMT_45Ka.gpr |
| GSM205267 |
13371173_532nm_720PMT_635nm_800PMT_45ka.gpr |
| GSM205268 |
13371177_532nm_720PMT_635nm_800PMT_45ka.gpr |
|
| Relations |
| BioProject |
PRJNA101215 |
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