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Series GSE18503 Query DataSets for GSE18503
Status Public on Oct 10, 2009
Title Effects of environmental chemicals on development of neuronal cell lineages derived from mouse ES cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study provides a comprehensive evaluation of changes in gene expression during treatment with environmental chemicals in embryonic bodies derived from mouse embryonic stem (ES) cells. ES cells were maintained under the feeder cells in phenol red-free DMEM Medium (supplemented with 15% FBS, 100uM Non-essential amino acids (NEAA), 1000U/ml Leukemia inhibitory factor(LIF), 100uM 2-mercaptoethanol(2-ME), and 0.5% penicillin/streptomycin). Cells were gently washed in warm PBS and transferred to phenol red-free DMEM Medium (supplemented with 15% KnockOut Serum Replacement, 100uM NEAA, 100uM 2-ME, and 0.5% penicillin/streptomycin) in Microsphere array (MSA300F) and exposed with low and high levels of each chemical just after removing LIF from the culture media. Prior to collection, cells were washed in warm PBS, resuspended and briefly incubated in the QIAGEN RLT buffer, and finally collected in triplicate at 48 hours. Following RNA isolation, the best RNA yields for each replicate set was selected for target preparation and microarray processing.
 
Overall design 25 total samples: 1 vehcle, 12 chemicals and two doses of each chemical [Vehicle Control (0.1% dimethyl sulfoxide), estradiol (1nM and 10nM), dehydrotestosterone (1nM and 10nM) bisphenol A (1pM and 100pM), triiodothyronine (1nM and 10nM), dexamethasone (1nM and 10nM), 2,3,7,8-tetrachlorodibenzo-p-dioxin (1nM and 10nM), 4(OH)-2’3,3’4’-5’-pentachlo biphenyls (1nM and 10nM), thalidomide (100nM and 0.01mM), bis(2-ethylhexyl)phthalate (0.001mM and 0.1mM), permethrin (100nM and 0.01mM), cyclopamine (100nM and 0.01mM), methoprene acid (0.001mM and 0.1mM)]; 1 Timepoint [48 hours].
 
Contributor(s) Sone H, Ohsako S
Citation(s) 22312247
Submission date Oct 09, 2009
Last update date Jan 16, 2019
Contact name Sone Hideko
E-mail(s) hsone@nies.go.jp
Phone 81298502420
Organization name National Institute for Environmental Studies
Department Center for Environmental Risk Research
Lab Environmental Exposure Research Section
Street address onogawa16-2
City tsukuba
State/province ibaraki
ZIP/Postal code 305-8506
Country Japan
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (25)
GSM461127 mES DMSO
GSM461128 mES E2-1nM
GSM461129 mES E2-10nM
Relations
BioProject PRJNA120253

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18503_RAW.tar 15.8 Mb (http)(custom) TAR
GSE18503_non-normalized.txt.gz 7.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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