"hdr_template" - PMC

Javier Fierro, Jr., Jake DiPasquale, Joshua Perez, Brandon Chin, Yathip Chokpapone, An M. Tran, Arabella Holden, Chris Factoriza, Nikhi Sivagnanakumar, Rocio Aguilar, Sarah Mazal, Melissa Lopez, Huanyu Dou

Sci Rep. 2022; 12: 2417. Published online 2022 Feb 14. doi: 10.1038/s41598-022-06430-1

PMCID:: PMC8844083

Abstract Article PDF–6.6M

Select item 69543104.

Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency

David N. Nguyen, Theodore L. Roth, P. Jonathan Li, Peixin Amy Chen, Ryan Apathy, Murad R. Mamedov, Linda T. Vo, Victoria R. Tobin, Daniel Goodman, Eric Shifrut, Jeffrey A. Bluestone, Jennifer M. Puck, Francis C. Szoka, Alexander Marson

Nat Biotechnol. Author manuscript; available in PMC 2020 Jun 9.

Published in final edited form as: Nat Biotechnol. 2020 Jan; 38(1): 44–49. Published online 2019 Dec 9. doi: 10.1038/s41587-019-0325-6

PMCID:: PMC6954310

Abstract Article PDF–1.5M

Select item 99475515.

CRISPR/Cas9-based Engineering of Immunoglobulin Loci in Hybridoma Cells

Camille Le M. Gall, Felix L. Fennemann, Johan M. S. Van Der Schoot, Ferenc A. Scheeren, Martijn Verdoes

Bio Protoc. 2023 Feb 20; 13(4): e4613. Published online 2023 Feb 20. doi: 10.21769/BioProtoc.4613

PMCID:: PMC9947551

Abstract Article PDF–6.4M

Select item 87783606.

Impact of Formulation Conditions on Lipid Nanoparticle Characteristics and Functional Delivery of CRISPR RNP for Gene Knock-Out and Correction

Johanna Walther, Danny Wilbie, Vincent S. J. Tissingh, Mert Öktem, Heleen van der Veen, Bo Lou, Enrico Mastrobattista

Pharmaceutics. 2022 Jan; 14(1): 213. Published online 2022 Jan 17. doi: 10.3390/pharmaceutics14010213

PMCID:: PMC8778360

Abstract Article PDF–3.1M

Select item 57850977.

CRISPR/Cas9 Mediated Fluorescent Tagging of Endogenous Proteins in Human Pluripotent Stem Cells

Arun Sharma, Christopher N. Toepfer, Tarsha Ward, Lauren Wasson, Radhika Agarwal, David A. Conner, Johnny H. Hu, Christine E. Seidman

Curr Protoc Hum Genet. Author manuscript; available in PMC 2019 Jan 24.

Published in final edited form as: Curr Protoc Hum Genet. 2018 Jan 24; 96: 21.11.1–21.11.20. Published online 2018 Jan 24. doi: 10.1002/cphg.52

PMCID:: PMC5785097

Abstract Article PDF–1.6M

Select item 57613248.

Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes

Ashley M. Jacobi, Garrett R. Rettig, Rolf Turk, Michael A. Collingwood, Sarah A. Zeiner, Rolen M. Quadros, Donald W. Harms, Paul J. Bonthuis, Christopher Gregg, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Mark A. Behlke

Methods. Author manuscript; available in PMC 2018 May 15.

Published in final edited form as: Methods. 2017 May 15; 121-122: 16–28. Published online 2017 Mar 27. doi: 10.1016/j.ymeth.2017.03.021

PMCID:: PMC5761324

Abstract Article PDF–901K

Select item 103232499.

Fast, precise and cloning-free knock-in of reporter sequences in vivo with high efficiency

Yiran Zhang, Katy Marshall-Phelps, Rafael Góis de Almeida

Development. 2023 Jun 15; 150(12): dev201323. Published online 2023 Jun 29. doi: 10.1242/dev.201323

PMCID:: PMC10323249

Abstract Article

Select item 623941710.

Reprogramming human T cell function and specificity with non-viral genome targeting

Theodore L. Roth, Cristina Puig-Saus, Ruby Yu, Eric Shifrut, Julia Carnevale, P. Jonathan Li, Joseph Hiatt, Justin Saco, Paige Krystofinski, Han Li, Victoria Tobin, David N. Nguyen, Michael R. Lee, Amy L. Putnam, Andrea L. Ferris, Jeff W. Chen, Jean-Nicolas Schickel, Laurence Pellerin, David Carmody, Gorka Alkorta-Aranburu, Daniela Del Gaudio, Hiroyuki Matsumoto, Montse Morell, Ying Mao, Min Cho, Rolen M. Quadros, Channabasavaiah B. Gurumurthy, Baz Smith, Michael Haugwitz, Stephen H. Hughes, Jonathan S. Weissman, Kathrin Schumann, Jonathan H. Esensten, Andrew P. May, Alan Ashworth, Gary M. Kupfer, Siri Atma W. Greeley, Rosa Bacchetta, Eric Meffre, Maria Grazia Roncarolo, Neil Romberg, Kevan C. Herold, Antoni Ribas, Manuel D. Leonetti, Alexander Marson

Nature. Author manuscript; available in PMC 2019 Jan 11.

Published in final edited form as: Nature. 2018 Jul; 559(7714): 405–409. Published online 2018 Jul 11. doi: 10.1038/s41586-018-0326-5

PMCID:: PMC6239417

Abstract Article PDF–2.3M

Select item 883590111.

Challenges of CRISPR-Based Gene Editing in Primary T Cells

Alaleh Rezalotfi, Lea Fritz, Reinhold Förster, Berislav Bošnjak

Int J Mol Sci. 2022 Feb; 23(3): 1689. Published online 2022 Feb 1. doi: 10.3390/ijms23031689

PMCID:: PMC8835901

Abstract Article PDF–1.4M

Select item 780829812.

A robust platform for expansion and genome editing of primary human natural killer cells

Rih-Sheng Huang, Min-Chi Lai, Hsin-An Shih, Steven Lin

J Exp Med. 2021 Mar 1; 218(3): e20201529. Published online 2021 Jan 12. doi: 10.1084/jem.20201529

PMCID:: PMC7808298

Abstract Article PDF–8.0M

Select item 610105213.

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Harriet Prior, Lauren MacConnachie, Jose L. Martinez, Georgina C.B. Nicholl, Asim A. Beg

J Vis Exp. 2018; (134): 57518. Published online 2018 Apr 30. doi: 10.3791/57518

PMCID:: PMC6101052

Abstract Article PDF–535K

Select item 726028214.

Condensin I is required for faithful meiosis in Drosophila males

Kristina Kleinschnitz, Nina Vießmann, Mareike Jordan, Stefan K. Heidmann

Chromosoma. 2020; 129(2): 141–160. Published online 2020 Apr 8. doi: 10.1007/s00412-020-00733-w

PMCID:: PMC7260282

Abstract Article PDF–2.5M

Select item 749136515.

ARF‐AID: A Rapidly Inducible Protein Degradation System That Preserves Basal Endogenous Protein Levels

Kizhakke Mattada Sathyan, Thomas G. Scott, Michael J. Guertin

Curr Protoc Mol Biol. 2020 Sep; 132(1): e124. Published online 2020 Aug 5. doi: 10.1002/cpmb.124

PMCID:: PMC7491365

Abstract Article PDF–4.2M

Select item 849625616.

DNA templates with blocked long 3ʹ end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci

Saptaparni Bandyopadhyay, Joseph Douglass, Sebastian Kapell, Nazimuddin Khan, Fabiana Feitosa-Suntheimer, Jenny A Klein, Jasmine Temple, Jayce Brown-Culbertson, Alexander H Tavares, Mohsan Saeed, Nelson C Lau

G3 (Bethesda) 2021 Aug; 11(8): jkab169. Published online 2021 May 14. doi: 10.1093/g3journal/jkab169

PMCID:: PMC8496256

Abstract Article PDF–1.4M

Select item 1002771517.

CRISPR/Cas9‐mediated homology donor repair base editing confers glyphosate resistance to rice (Oryza sativa L.)

Sonia Khan Sony, Tanushri Kaul, Khaled Fathy Abdel Motelb, Arulprakash Thangaraj, Jyotsna Bharti, Rashmi Kaul, Rachana Verma, Mamta Nehra

Front Plant Sci. 2023; 14: 1122926. Published online 2023 Mar 7. doi: 10.3389/fpls.2023.1122926

PMCID:: PMC10027715

Abstract Article PDF–17M

Select item 843150718.

Efficient Generation of Knock-In Zebrafish Models for Inherited Disorders Using CRISPR-Cas9 Ribonucleoprotein Complexes

Erik de Vrieze, Suzanne E. de Bruijn, Janine Reurink, Sanne Broekman, Vince van de Riet, Marco Aben, Hannie Kremer, Erwin van Wijk

Int J Mol Sci. 2021 Sep; 22(17): 9429. Published online 2021 Aug 30. doi: 10.3390/ijms22179429

PMCID:: PMC8431507

Abstract Article PDF–1.4M

Select item 761193419.

A compact Cascade-Cas3 system for targeted genome engineering

Bálint Csörgő, Lina M. León, Ilea J. Chau-Ly, Alejandro Vasquez-Rifo, Joel D. Berry, Caroline Mahendra, Emily D. Crawford, Jennifer D. Lewis, Joseph Bondy-Denomy

Nat Methods. Author manuscript; available in PMC 2021 Nov 3.

Published in final edited form as: Nat Methods. 2020 Dec 1; 17(12): 1183–1190. Published online 2020 Oct 19. doi: 10.1038/s41592-020-00980-w

PMCID:: PMC7611934

Abstract Article PDF–1.4M

Select item 1022566920.

Reprogramming Mycobacterium tuberculosis CRISPR System for Gene Editing and Genome-wide RNA Interference Screening

Khaista Rahman, Muhammad Jamal, Xi Chen, Wei Zhou, Bin Yang, Yanyan Zou, Weize Xu, Yingying Lei, Chengchao Wu, Xiaojian Cao, Rohit Tyagi, Muhammad Ahsan Naeem, Da Lin, Zeshan Habib, Nan Peng, Zhen F. Fu, Gang Cao

Genomics Proteomics Bioinformatics. 2022 Dec; 20(6): 1180–1196. Published online 2021 Dec 16. doi: 10.1016/j.gpb.2021.01.008

PMCID:: PMC10225669

Abstract Article PDF–3.9M

PMC

Result Filters

Article attributes

Text availability

Publication date

Custom date range

Research Funder

Additional filters

Display Settings:

Send to:

PMC Full-Text Search Results

Items: 1 to 20 of 900

Display Settings:

Send to:

Supplemental Content

Find related data

Search details

Recent activity