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Inhibition of Trypanosoma cruzi Sterol 14-alpha demethylase
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of C-terminal His6-tagged Trypanosoma cruzi CYP51 expressed in Escherichia coli HMS174(DE3) assessed as dissociation constant by spectrophotometry
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Displacement of [3H]-Eubricol from C-terminal His-tagged full-length Trypanosoma cruzi Tulahuen strain CYP51 expressed in Escherichia coli HMS174(DE3) assessed as dissociation constant preincubated for 5 mins measured after 60 mins by enzymatic assay
Inhibition of recombinant Trypanosoma cruzi Tulahuen strain CYP51 expressed in Escherichia coli JM109
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
CYP51 Inhibition Assay from Article 10.1074/jbc.M113.497990: "Complexes of Trypanosoma cruzi sterol 14a-demethylase (CYP51) with two pyridine-based drug candidates for Chagas disease: structural basis for pathogen selectivity."
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Inhibition Assay from Article 10.1016/j.chembiol.2007.10.011: "Sterol 14alpha-demethylase as a potential target for antitrypanosomal therapy: enzyme inhibition and parasite cell growth."
Assay data:17 Active, 16 Activity ≤ 1 µM, 17 Tested
Spectral Titration of TCCYP51 from Article 10.1021/jm801643b: "Indomethacin amides as a novel molecular scaffold for targeting Trypanosoma cruzi sterol 14alpha-demethylase."
Assay data:13 Active, 10 Activity ≤ 1 µM, 13 Tested
Inhibition of Trypanosoma cruzi CYP51
Assay data:4 Active, 3 Activity ≤ 1 µM, 4 Tested
Binding affinity to Trypanosoma cruzi CYP51 at 5 to 10 uM by UV-vis spectrophotometry
Assay data:4 Active, 4 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to Trypanosoma cruzi CYP51 by UV-vis spectrophotometry
Assay data:1 Active, 1 Tested
Inhibition of Trypanosoma cruzi Tulahuen C4 CYP51 expressed in Escherichia coli HMS174 using [3H]-sterol as substrate in presence of NADPH by reversed phase HPLC analysis
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to full length Trypanosoma cruzi Tulahuen C4 C-terminal His-tagged CYP51 expressed in Escherichia coli HMS174 (DE3) assessed as induction of shift in Soret band by spectrophotometry
Assay data:2 Active, 1 Activity ≤ 1 µM, 3 Tested
Binding affinity to Trypanosoma cruzi CYP51 by spectrophotometric method
Inhibition of Trypanosoma cruzi CYP51 assessed as residual enzyme activity at 100 uM using [3H]-MDL as substrate pretreated for 10 mins followed by NADPH addition by RP-HPLC analysis relative to control
Assay data:6 Tested
Inhibition of Trypanosoma cruzi CYP51 assessed as residual enzyme activity at 100 uM using [3H]-MDL as substrate pretreated for 5 mins followed by NADPH addition by RP-HPLC analysis relative to control
Binding affinity to Trypanosoma cruzi Y CYP51A expressed in Escherichia coli
Inhibition of Trypanosoma cruzi Y CYP51 expressed in Escherichia coli using [3-3H]-eburicol as substrate after 60 mins by LC-MS analysis
Assay data:4 Active, 5 Tested
Inhibition of Trypanosoma brucei Tulahuen CYP51 expressed in Escherichia coli using [3-3H]-obtusifoliol as substrate after 60 mins by LC-MS analysis
Binding affinity to Trypanosoma cruzi CYP51 by quartz cuvette based UV-vis spectroscopic method
Binding affinity to Trypanosoma cruzi CYP51 at 5 to 10 uM by 96-well based UV-vis spectroscopic method
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