16S v3v4 of Equus caballus: feces stored at -20 - SRA

SRX9507891: 16S v3v4 of Equus caballus: feces stored at -20
1 ILLUMINA (Illumina MiSeq) run: 98,309 spots, 59M bases, 40.4Mb downloads

Design: Using 2 mL bead beating tubes (0.7 mm Dry Garnet) and a Vortexed-Genie 2 fitted with Qiagens Vortex Adapter (cat. No. 13000-V1-24), we homogenized 0.20-gram sub-samples of horse fecal material. We extracted DNA from homogenized fecal samples using QIAgens QIAamp PowerFecal DNA Kits, following manufacturer recommendations outlined in the Qiagen PowerFecal DNA handbook. Notably, we used a single tube extraction protocol (rather than a 96-well format) and randomized the order in which samples were extracted. In the final step, we eluted DNA from the spin columns using 100 l of ddH2O pre-warmed to 60C. Prior to sequencing, we quantified the DNA in eluted extracts using a Qubit dsDNA BR Assay Kit and standardized DNA concentration to 20 ng/uL prior to PCR amplification. We PCR amplified the v3v4 region of the 16S rRNA gene using the 341f forward and 805r reverse universal primers. PCR products were sequenced on an Illumina MiSeq platform (v3 chemistry: 2 x 300 base-pair read pairs) at the University of Calgary Centre for Health Genomics and Informatics. Feces samples stored at -20 degrees celsius prior to DNA extraction

Submitted by: University of Calgary

Study: Sable Island horse fecal bacterial microbiome 16S sequences

PRJNA674675 • SRP291220 • All experiments • All runs

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Bacterial 16S amplicon sequences generated from fecal samples collected from 86 adult mares from a population of feral horses on Sable Island Nova Scotia. The objective of the study was to characterize the Sable Island horse fecal microbiome and to parse the effects of local environmental and spatial processes.

Sample:

SAMN16668277 • SRS7653649 • All experiments • All runs

Organism: feces metagenome

Library:

Name: SI_2014_16S_S146_minus20

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 98,309 spots, 59M bases, 40.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR13059746	98,309	59M	40.4Mb	2020-12-17

ID:: 12424156

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